Juventus lost after Genoa condemned Massimiliano Allegri's side to a shock 2-1 defeat.
Juventus have already qualified for next season's Champions League, remaining fourth on 69 points and with two games left to play.
They can no longer overtake Inter Milan (78) at the top of the standings.
Friday's loss marks Juventus' second defeat in their last 22 games.
Juve barely threatened Genoa's goal in the first half, sending only one shot on target in 45 minutes.
Genoa are 19th with 28 points, just one point away from the safety zone with two games remaining.
Source: ESPN